Submit Manuscript  

Article Details

Analysis of Annulated Sea Snake Venom, Hydrophis Cyanocinctus, Using Liquid Chromatography and MALDI-TOF/TOF

[ Vol. 12 , Issue. 1 ]


Saeedeh Mosafer Khorjestan, Behrooz Abtahi, Seyed Omid Ranei Siadat, Mohammad Motevalli, Hassan Rezadoost, Parviz Ghezellou and Alireza Ghassempour   Pages 45 - 55 ( 11 )


Annulated sea snake, Hydrophis cyanocinctus, venom was investigated using reversed-phase high-performance liquid chromatography coupled to electrospray mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF/TOF). MALDI-TOF/TOF identified three compounds (a short-chain neurotoxin, a long-chain neurotoxin and a basic phospholipase A2); whose molecular weights were estimated to be 7285, 10838, and 14235 Da, respectively. Moreover, evolutionary relationship of each protein (identity more than 70%) was separately investigated via phylogenetic trees. This study was done as complementary investigation on the composition of the venom of H. cyanocinctus, the annulated sea snake from the Persian Gulf.


Persian Gulf, phospholipase A2, sea snake, three-finger toxin, venom.


Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C. Evin, Tehran, Iran.

Graphical Abstract:

Read Full-Text article