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Secretome Analysis of a Pseudomonas aeruginosa Strain Grown Under High Alkane Stress

[ Vol. 12 , Issue. 3 ]

Author(s):

R. Hemamalini and Sunil Kumar Khare   Pages 202 - 208 ( 7 )

Abstract:


The effect of alkanes, viz. n-hexane, n-heptane and n-octane on the extracellular proteome and secretome of a solvent tolerant Pseudomonas aeruginosa has been described. Superoxide dismutase, GroEL, A-type flagellin and the porin OprF were altered significantly. The presence of these proteins in the extracellular proteome of solvent-grown cells indicates their role in the cellular adaptation of the strain to a high alkane environment. Up regulation of superoxide dismutase indicates creation of oxidative stress for the bacterial cell in an alkane-rich environment. This secretome analysis is useful for gaining a better understanding of the solvent mediated oxidative stress and the role of superoxide dismutase and GroEL expression as possible markers of solvent tolerance.

Keywords:

Elastase, endopeptidase, OprF, GroEL, superoxide dismutase, solvent tolerance.

Affiliation:

Enzyme and Microbial Biochemistry Lab, Department of Chemistry, Indian Institute of Technology, Delhi, New Delhi, India.

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