R. Hemamalini and Sunil Kumar Khare Pages 202 - 208 ( 7 )
The effect of alkanes, viz. n-hexane, n-heptane and n-octane on the extracellular proteome and secretome of a solvent tolerant Pseudomonas aeruginosa has been described. Superoxide dismutase, GroEL, A-type flagellin and the porin OprF were altered significantly. The presence of these proteins in the extracellular proteome of solvent-grown cells indicates their role in the cellular adaptation of the strain to a high alkane environment. Up regulation of superoxide dismutase indicates creation of oxidative stress for the bacterial cell in an alkane-rich environment. This secretome analysis is useful for gaining a better understanding of the solvent mediated oxidative stress and the role of superoxide dismutase and GroEL expression as possible markers of solvent tolerance.
Elastase, endopeptidase, OprF, GroEL, superoxide dismutase, solvent tolerance.
Enzyme and Microbial Biochemistry Lab, Department of Chemistry, Indian Institute of Technology, Delhi, New Delhi, India.