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Proteomic Analysis of Two Rice Varieties with Contrasting Drought Tolerance at Reproductive Stage

[ Vol. 13 , Issue. 3 ]

Author(s):

Wei Huang, Ting Bi, Fangjun Feng, Xiaosong Ma, Jing Pan, Qiaojun Lou, Hanwei Mei and Weining Sun   Pages 218 - 230 ( 13 )

Abstract:


Drought is a major environmental factor that limits grain yield in rice. In order to understand the proteomic changes in response to drought at reproductive stage, proteins expressed in the young panicles were compared between two varieties of rice with contrasting tolernace to drought. The identified 36 differentially expressed proteins are involved in energy production, metabolism, stress defense, protein destination and cell structure. A coordinative up-regulation of triosephosphate isomerase, glycin decarboxylase complex H-protein, glyoxylase I and cytosolic ascorbate peroxidase was obseved in the drought tolerant variety Zhonghan 3, which may contribute to higher tolerance by increasing the energy production and maintaining redox homeostasis. Two-dimensional Western blotting experiments identified three APX1 and three APX2 isoforms up-regulated during drought stress in drought-tolerant varieties (Zhonghan 3, IRAT109 and PRATAO). However, in droughtsuceptible varieties (IR29, Zhengshan 97B and CATUNA), expression of the six isoforms was unchanged or even down-regulated. Under normal growth conditions, expression level of the six APX isoforms showed negative correlation with drought tolerance of rice. Our data suggested that APX isoforms may be considered as indicators of the redox status that related to drought tolerance in rice.

Keywords:

Cytosolic ascorbate peroxidase, drought tolerance, panicle, proteome, redox, rice.

Affiliation:

Institute of Plant Physiology and Ecology, SIBS, CAS, Shanghai, China; Present Address: State Key Laboratory of Genetic Engineering, Institute of Plant Biology, Center for Evolutionary Biology, School of Life Sciences, Fudan University, Shanghai 200433, China.

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