Lei Pan and Peifeng Chen* Pages 138 - 145 ( 8 )
Objectives: We have analyzed the influence of Amorphophallus konjac tuber (KONJAC) extracts on protein expression of mitochondrial in cell line SGC-7901, identified those differentially expressed by mass spectrometry, and built a network of differentially expressed proteins using software. This provided the basis for a study of the molecular mechanisms of the anti-gastric cancer effects.
Method: The cell viability was evaluated by cell counting kit-8. Cell apoptosis assay was used to detect cell apoptosis. Two-dimensional gel electrophoresis and MALDI-TOF-MS analysis was applied to identify the differentially-expressed mitochondrial protein treatment with or without KONJAC extracts. Ingenuity Pathways Analysis (IPA) software was to analysis an interacting protein.
Result: Proliferation of SGC-7901 cells was reduced and early apoptosis increased after treatment with KONJAC extracts. Two-dimensional gel electrophoresis and MALDI-TOF-MS analysis showed that 682 protein spots in control group and 643 protein spots in the KONJAC extract treated group. Twenty proteins were identified that showed 10 up-regulated and 10 down-regulated proteins in these arrays. Bioinformatic IPA network analysis showed that ubiquitin-conjugating enzyme 2(E2) was located in the center of the network. Thus, the inhibition of cell proliferation and promotion of apoptosis by KONJAC extracts may be through ubiquitin conjugating enzyme-mediated mitochondrial apoptotic pathways.
Conclusion: These results indicate that KONJAC extract-induced apoptosis and inhibit proliferation in SGC-7901 cells is mediated by pathways involving in mitochondrial apoptotic, gene transcription, cell cycle, oxidative stress response and energy metabolism. KONJAC extracts could be promising as a treatment for gastric cancer.
Amorphophallus konjac, gastric cancer, mitochondrial protein, proteomics, tuber extracts.
The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, First Affiliated Hospital of Zhejiang Chinese Medical University, 54 Youdian Road, Hangzhou, Zhejiang 310006