Article Details


In Silico Study of Different Signal Peptides for Secretory Production of Interleukin-11 in Escherichia coli

[ Vol. 14 , Issue. 2 ]

Author(s):

Manica Negahdaripour, Navid Nezafat, Nasim Hajighahramani, Seyyed Soheil Rahmatabadi, Mohammad Hossein Morowvat and Younes Ghasemi   Pages 112 - 121 ( 10 )

Abstract:


Background: Secretory production of heterologous proteins in bacterial hosts has been a topic of interest due to its various advantages. However, it is difficult to achieve because of process complexity and the need for selection of an appropriate signal peptide for each protein and host. IL- 11, marketed as Neumega®, is a multifunctional cytokine approved for platelet recovery in chemotherapy- induced thrombocytopenia.

Objective: The in silico evaluation of 30 signal peptides for finding the best theoretical candidates for the secretory production of recombinant IL-11 in Escherichia coli.

Methods: The prediction of signal peptide presence and location of cleavage sites were done by SignalP 4.1. Five signal peptides (ompT, npr, TorA, caf1M, and phoA) were excluded from further evaluations due to cleavage issues. Different physicochemical properties of signal peptides, which may affect protein secretion, were studied by ProtParam and PROSO II servers.

Results/Conclusion: Computational analysis of the influencing factors identified TorT, sfmC, and ompC, respectively, as the best theoretical candidates for the secretory production of IL-11 in E. coli. However, in the experimental investigation, other influencing factors and a system biology approach should be considered.

Keywords:

Interleukin-11, in silico, oprelvekin, secretory production, signal peptide, SRP-pathway.

Affiliation:

Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Pharmaceutical Sciences Research Center, School of Pharmacy, Shiraz University of Medical Science, Shiraz, Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Pharmaceutical Sciences Research Center, School of Pharmacy, Shiraz University of Medical Science, Shiraz, Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz

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