Noemí M. Atucha, Paola Romecin, Francisco Solano and Joaquín García-Estañ* Pages 234 - 241 ( 8 )
Background: Hepatic cirrhosis is associated with a variety of hemodynamic and hydrodynamic abnormalities, including renal dysfunction with salt and water retention leading to ascites and dilutional hyponatremia.Objective: In the present study, a proteomic analysis of the kidney of rats subjected to bile duct ligation (BDL) has been carried out in order to identify the proteins altered under this condition. Method: The experiments were performed at the fourth week after BDL, and the protein mixtures were separated by two-dimensional gel electrophoresis using the differential in-gel system (DIGE). An internal standard composed of equal amounts of the above 12 extracts was labeled with Cy2, and loaded onto six gels. Results: More than 4,000 proteins were labeled and numbered, and around 60 proteins were differentially expressed in the kidneys of BDL rats as compared to the controls, as analyzed by De- Cyder software. The respective spots were excised and digested with trypsin for MALDITOF/ TOF peptide mass fingerprinting analysis. Some of the proteins down-regulated in the kidney of the BDL rats were Ezrin, Moesin and Regucalcin, whereas Annexin-4, Cathepsin B and Heat Shock Proteins were significantly up-regulated. Discussion: The relationship of these proteins to the systemic and renal abnormalities seen in BDL rats is discussed.
Sodium retention, ascites, cholestasis, renal proteomics, renal proteins, renal biomarkers, liver cirrhosis.
Dpto. Fisiología, Facultad de Medicina, Instituto Murciano de Investigación Biosanitaria (IMIB), Universidad de Murcia, Dpto. Fisiología, Facultad de Medicina, Instituto Murciano de Investigación Biosanitaria (IMIB), Universidad de Murcia, Dpto. Bioquímica y Biología Molecular B, Facultad de Medicina, Instituto Murciano de Investigación Biosanitaria (IMIB), Universidad de Murcia, Depto. Fisiologia, Fac. Medicina, Campus de Ciencias de la Salud, B2.3.032, 30120 El Palmar, Murcia