Zhen Yang*, De-zhao Lu and Lihua Tang Pages 55 - 61 ( 7 )
Background: EGCG, (-)-epigallocatechin-3-gallate, a constituent of catechin with the highest content in green tea, is currently show anti-atherogenic effects ex vivo in humans.
Objective: To study the effect of EGCG on proteome of Raw264.7 macrophage-derived foam cell by using two-dimensional electrophoresis and mass spectrum.
Method: The proteins from macrophage cells, foam cells, and EGCG-treated cells with 30µg/mL EGCG were separated with two-dimensional electrophoresis and the gels were scanned after silver staining. All images were analyzed by Image Master 2D 6.0 software, and the different proteins were identified by mass spectrum.
Results: M2-type pyruvate kinase, glucose-6-phosphatase, triose phosphate isomerase, ATP synthase, stathmin 1, proteasome 26S, ubiquitin-conjugating enzyme, ring finger protein 130, glyoxalase domain containing 4, beta-actin, Tropomyosin, A-X actin, heat shock protein 70, Adenine phosphoribosyltransferase, macrophage migration inhibitory factor 13kda protein, and anti-sense basic fibroblast growth factor, were identified to be differentially expressed among the macrophage cells, foam cells, and EGCG-treated foam cells.
Conclusion: EGCG prevents the development of atherosclerosis probably through changing the expression of cytoskeletal proteins and antioxidant enzymes to regulate the energy metabolism in foam cells.
Cholesterol balance, EGCG, mass spectrum, oxidative modification LDL, proteome, Raw264.7 macrophage cell.
College of Life Science, Zhejiang Chinese Medical University, Hangzhou, 310053, College of Life Science, Zhejiang Chinese Medical University, Hangzhou, 310053, College of Life Science, Zhejiang Chinese Medical University, Hangzhou, 310053