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Identification of Secretome of MRC-5 Fibroblast Cell Line Using Two-dimensional Electrophoresis Coupled to Mass Spectrometry

[ Vol. 15 , Issue. 1 ]

Author(s):

Zahra Mojtahedi, Kazuyuki Nakamura, Yasuhiro Kuramitsu, Abbas Ghaderi and Jamal Sarvari*   Pages 77 - 82 ( 6 )

Abstract:


Objective: Secreted proteins from fibroblasts critically contribute to tumor microenvironment. Human embryonic lung fibroblast MRC-5cell line is a widely used model in cancer formation and progression studies. However, data regarding its secretome are poor.

Method: In the present study, we used immobilized pH gradient-based two dimensional gel electrophoresis (2DE) and mass spectrometry to characterize the secretome of MRC-5 cell line.

Results: In total, 21 protein spots related to 17 proteins were identified. Some of them were insulin-like growth factor-binding protein 5, epididymal secretory protein E1 (Niemann-Pick disease type C2 protein), cathepsin L, glutathione S-transferase P, ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5), UPF0587 protein C1orf123, transgelin, cofilin-1, cofilin-2, and guanine nucleotide-binding protein subunit beta-2-like 1.

Conclusion: Using 2DE and mass spectrometry, we identified several proteins in the conditioned media of MRC-5 cell line. The identified proteins were known to be involved in the key cellular processes. Our findings shed more light on the proteome mapping of secreted proteins from a fibroblast cell line.

Keywords:

Biomarkers, cancer, mass spectrometry, MRC-5, secretome, two-dimensional electrophoresis.

Affiliation:

Cancer Biomarkers and Proteomics Lab, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz 71348, Department of Biochemistry and Functional Proteomics, Yamaguchi University, Graduate School of Medicine, Yamaguchi 7538511, Department of Biochemistry and Functional Proteomics, Yamaguchi University, Graduate School of Medicine, Yamaguchi 7538511, Cancer Biomarkers and Proteomics Lab, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz 71348, Cancer Biomarkers and Proteomics Lab, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz 71348

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