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Differentially Expressed Wound-response-related Proteins from a Major Agarwood-producing Tree, Aquilaria malaccensis Lam. Identified via 2-D Electrophoresis

[ Vol. 15 , Issue. 4 ]

Author(s):

Shiou Yih Lee, Samsuddin Ahmad Syazwan, Dhilia Udie Lamasudin and Rozi Mohamed*   Pages 291 - 298 ( 8 )

Abstract:


Background: Wounding is the major trigger factor for agarwood formation in Aquilaria malaccensis. To date, the mechanism of agarwood formation in Aquilaria has been extensively explored through anatomy and genomics approaches; however, at the protein level, it is yet to be reported.

Objective: A comparative proteomics analysis was carried out to identify proteins responsive to wounding in A. malaccensis, using two-dimensional gel electrophoresis (2-DE) coupled with Matrix- Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).

Method: Proteins were extracted from A. malaccensis tree stems at 0, 6, 12, and 24 h after wounding and separated by 2-DE, sequenced using MALDI-TOF MS and identified through UniProt/Swiss-Prot protein database.

Results: Changes in differentially displayed proteins were observed. A total of 15 protein spots were identified reproducible under 2-DE, of which only two protein spots showed differences in expression affected by the wounding treatment in a time-dependent experiment. They were predicted as Malate Synthase (MS) and the reduced Nicotinamide Adenine Dinucleotide Phosphate (NADPH) quinone oxidoreductase subunit 2 B. Both proteins were directly and indirectly related to wounding treatments in plants and may be related to agarwood formation mechanism.

Conclusion: As Aquilaria proteome is not available to date, this first report on differential protein expression in response to wounding could serve as a useful information towards understanding agarwood biosynthesis at the protein level.

Keywords:

Agarwood, gaharu, 2-DE, MALDI-TOF, proteome, electrophoresis, wound.

Affiliation:

Forest Biotech Laboratory, Department of Forest Management, Faculty of Forestry, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Forest Biotech Laboratory, Department of Forest Management, Faculty of Forestry, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Halal Products Research Institute, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Forest Biotech Laboratory, Department of Forest Management, Faculty of Forestry, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor

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