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Effect of Intestinal Flora Clearance on Liver Proteomics in Mice

[ Vol. 16 , Issue. 3 ]

Author(s):

Zhenghu Jia*, Hui Liu, Mei Song, Chengmao Yang, Yapu Zhao, Xiaoli Wu, Zhenzhou Wu and Liqing Zhao   Pages 199 - 209 ( 11 )

Abstract:


Background: Intestinal flora dynamically affects the host's systemic immune system. Liver is one of the organs that may be affected by intestinal microbiota.

Materials and Methods: In this study, we aimed to identify proteome level differences between liver tissue from mice cleared intestinal flora and control using tandem mass spectrometry (LC-MS/MS) and label free quantification. Additionally, protein-protein interactions were mapped by STRING, and also, the enrichment of inflammation-related signaling pathways and biological processes was identified using GO and IPA network system. RT-PCR and Western blot were used for validation of the proteomics findings.

Results: Our study demonstrated that mice with cleared intestinal flora exhibited decreased sensitivity to Concanavalin A induced acute hepatitis. 324 proteins in liver were differently expressed after intestinal flora clearance for one week while 210 proteins were differently expressed after intestinal flora clearance for two weeks. Furthermore, five of the identified proteins were validated by western blotting and further investigated by semi-quantitative RT-PCR.

Conclusion: Our results showed that intestinal flora clearance in mice could reduce sensitivity to Concanavalin A induced liver injury and influence the expression of proteins in liver, which provides a clue for studying the relationship between gut bacteria and Concanavalin A induced hepatitis.

Keywords:

Intestinal flora, proteomics, Concanavalin A induced liver injury, hepatitis, injury, formic acid.

Affiliation:

State Key Laboratory of Medicinal Chemical Biology (Nankai University), College of Life Sciences, Nankai University, Tianjin, 300071, State Key Laboratory of Medicinal Chemical Biology (Nankai University), College of Life Sciences, Nankai University, Tianjin, 300071, State Key Laboratory of Medicinal Chemical Biology (Nankai University), College of Life Sciences, Nankai University, Tianjin, 300071, State Key Laboratory of Medicinal Chemical Biology (Nankai University), College of Life Sciences, Nankai University, Tianjin, 300071, People`s Liberation Army No. 254 Hospital, Tianjin, 300142, School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro Nano Biomaterials and Detection Treatment Technology, and Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, 300072, State Key Laboratory of Medicinal Chemical Biology (Nankai University), College of Life Sciences, Nankai University, Tianjin, 300071, State Key Laboratory of Medicinal Chemical Biology (Nankai University), College of Life Sciences, Nankai University, Tianjin, 300071



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