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Analysis of Extracellular Proteome of Staphylococcus aureus: A Mass Spectrometry based Proteomics Method of Exotoxin Characterisation

[ Vol. 17 , Issue. 1 ]

Author(s):

Rajdeep Das, Nisha D`souza, Surya Kant Choubey, Sethumadhavan Murlidharan, Anura V. Kurpad and Amit Kumar Mandal*   Pages 3 - 9 ( 7 )

Abstract:


Background: Staphylococcus aureus (S. aureus), an important pathogen, causes a wide range of infections in human starting from food poisoning to septicemia. It affects the host cells with various exotoxins, known as virulence factors, which are synthesized in growth phase-dependent manner of the bacteria. S. aureus has been reported to become resistant to antibiotics rapidly. Among two common clinical isolates, Methicillin-sensitive S. aureus (MSSA) and Methicillin-resistant S. aureus (MRSA), MRSA pose major problems across hospitals around the world.

Objective: The objective of the present study was to profile the exoproteins of Methicillin-sensitive S. aureus (ATCC 25293) and subsequently to establish a proteomics-based method of characterization of S. aureus that is crucial in treating hospital-acquired infections.

Method: We used two-dimensional nanoLC/ESI-MS based proteomic platform to characterize and quantify the exoproteins isolated from Methicillin-sensitive S. aureus (ATCC 25293) strain.

Results: A total of 69 proteins were identified from extracellular proteome pool of ATCC 25293 strain that includes 18 extracellular proteins, 40 cytoplasmic proteins, 2 membrane proteins, 3 cell wall proteins and 6 uncharacterized proteins.

Conclusion: We propose that this mass spectrometry-based proteomics method of characterization of exoproteins might be useful to identify S. aureus strains that are resistant to antibiotics.

Keywords:

Staphylococcus aureus, extracellular proteome, mass spectrometry, nanoLC, toxin, antibiotics.

Affiliation:

Clinical Proteomics Unit, Division of Molecular Medicine, St. John's Research Institute, St. John's National Academy of Health Sciences, Bangalore, 560034, Clinical Proteomics Unit, Division of Molecular Medicine, St. John's Research Institute, St. John's National Academy of Health Sciences, Bangalore, 560034, Department of Microbiology, St. John�s Medical College, St. John's National Academy of Health Sciences, Bangalore, 560034, Department of Microbiology, St. John`s Medical College, St. John`s National Academy of Health Sciences, Bangalore, Department of Physiology, St. John�s Medical College, St. John's National Academy of Health Sciences, Bangalore, 560034, Clinical Proteomics Unit, Division of Molecular Medicine, St. John's Research Institute, St. John's National Academy of Health Sciences, Bangalore, 560034



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