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Proteomics Study of Mesenchymal Stem Cell-Like Cells Isolated from Cerebrospinal Fluid of Patients with Meningioma

[ Vol. 16 , Issue. 4 ]

Author(s):

Arash Saffarian, Amir Tarokh, Mohammad Reza Haghshenas, Mousa Taghipour, Nooshafarin Chenari, Abbas Ghaderi and Mahboobeh Razmkhah*   Pages 282 - 288 ( 7 )

Abstract:


Background: Cerebrospinal fluid (CSF) contains pro-growth factors that can affect proliferation, migration and differentiation of Mesenchymal Stem Cells (MSCs).

Objective: This study aimed to isolate MSC like cells from CSF of patients with meningioma and psudotumorcerebri (PTC) and identify differentially expressed proteins in these cells.

Methods: Five patients with newly diagnosed intracranial meningioma and five patients with PTC were recruited in this comparative proteomics study. MSCs were isolated from CSF and validated by mesenchyml and non-mesenchyml fluorochrome antibodies, and flow cytometer analysis. Two- Dimensional Gel Electrophoresis (2-DE) coupled with Mass Spectrometry (MS) was performed to identify differentially expressed proteins.

Results: Microscopic views of the isolated cells as well as flow cytometer analysis were found to be compatible with MSC-like cells. Eight distinct protein spots were differentially and reproducibly expressed among the stained gels of two studied groups. The identified proteins were Phosphoglycerate Mutase 1 (PGAM1), LIM and SH3 domain protein (LASP1), peroxiredoxin-6 (PRDX-6), type I cytoskeletal 9 (KRT9), Superoxide Dismutase (SOD), endoplasmin, Stathmin 1 (STMN1), and glutathione S-transferase (GST).

Conclusion: This study provides new insights into the plausible role of CSF derived MSCs in cancer progression, and reveals a promising therapeutic opportunity for targeting of MSC proteins in patients with meningioma.

Keywords:

Meningioma, psudotumorcerebri, cerebrospinal fluid, mesenchymal stem cells, proteomics, staining buffer.

Affiliation:

Department of Neurosurgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Department of Neurosurgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Department of Neurosurgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz

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