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Comparative iTRAQ Proteomic Analysis Provides the Molecular basis of the Metabolites in Cultivated Glycyrrhiza uralensis and its Wild-type

Author(s):

Chengcheng Wang, Lihong Chen , Zhichen Cai , Sijing Feng , Moyi Yue , Jiaojiao Yang , Lisi Zou and Xunhong Liu *   Pages 1 - 14 ( 14 )

Abstract:


Background: Licorice is an extensively applied herbal medicine around the world and Glycyrrhiza uralensis Fisch provides most of licorice resources for clinical consumption. Evidence suggested there was significant difference in metabolite composition of licorice due to its ecotypes.

Object: To better understand the proteomic changes and molecular mechanism of metabolites formation between wild Glycyrrhiza uralensis Fisch and its cultivated-type.

Method: Firstly, we established proteome database by annotating protein sequences according to the genomic and transcriptomic data of G. uralensis. Then iTRAQ - LC-MS/MS technologies were applied to detect significantly statistical protein changes between cultivated and wild G. uralensis. 2751 validated proteins were obtained with high confidence and 333 were found with differential abundance. Differentially expressed proteins were identified and were analyzed by GO, KEGG, string network and pathway enrichment. Ultimately, we combined results of iTRAQ with our previous investigation on metabolites in order to underlie the molecular mechanism of metabolite accumulation.

Result: Results showed that differentially expressed proteins were mainly involved in the anabolism of carbohydrate and important amino acids, which participated in life activities and secondary metabolite synthesis. Another important pathway is the synthesis of flavonoids which is generally accepted as important bioactive constituents in G. uralensis and the accumulation of flavonoids in different synthetic stages is diverse in two ecotypes of G. uralensis. Therefore, proteins with unbalanced level present throughout the wild and cultivated G. uralensis possibly resulted in differences of medicinal compounds.

Conclusion: Our study will provide a novel clue for revealing the molecular mechanism of differences in secondary metabolites as well as quality formation in wild and cultivated G. uralensis.

Keywords:

Glycyrrhiza uralensis Fisch, Wild-type, Cultivated type, secondary metabolites, flavonoids, iTRAQ

Affiliation:

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023



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