Sonia Kumari Shishodia and Jata Shankar* Pages 1 - 13 ( 13 )
Background: Aspergillus terreus is a major etiological agent among fungal pathogens and emerged as an opportunistic pathogen in immunocompromised patients. Objective: To explore mycelial proteins to shed light on chemical and molecular details that contribute pathogenesis including intrinsic resistant to amphotericin B (AmB), and biomolecules associated with biofilm formation.
Methods: The mycelial proteins of A. terreus cultured for 48h at 37°C have been analysed by using nLC-ESI-MS/MS. Protein data analysis performed by Proteome Discoverer (V/2.2) against the UniProt database. Also, scanning electron microscopy (SEM) analysis of biofilm formation under optimum conditions were carried out. qRT-PCR was carried for genes encoding for functionally important proteins.
Results: A total of 389 proteins were detected at FDR 0.01. Gene ontology showed the abundance of proteins from energy metabolism, cellular homeostasis, ribosome biogenesis, cell wall and structural components. We observed catalase, superoxide dismutase, thioredoxin, glutathione S-transferase involved in redox homeostasis and heat shock proteins (Hsp90 and Hsp70). These proteins may provide insight into the resistance mechanism of A. terreus against AmB. Additionally, SEM analysis of A. terreus showed formation of dense hyphal network covered with porous extracellular matrix (ECM). Using SECRETOOL, 8 proteins were predicted as secretory. Three proteins such as glucanase Crf1/allergen (Asp F9), 1, 3-β-glucanosyltransferase and β-hexosaminidase were reported in biofilm establishment.
Conclusion: Our proteome data provided experimental evidence to annotated set of proteins in A. terreus that comprehend biochemical and cellular events involved in the establishment of mycelial network and contributing to the pathogenesis. This work also provides resource for further molecular studies in A. terreus
Amphotericin B resistance, Extracellular matrix, Secretory proteins, Adhesion, Biofilm
Genomics laboratory, Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan-173234, Himachal Pradesh, Genomics laboratory, Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan-173234, Himachal Pradesh