Nafeesa Khatoon, Hammad Alam and Meryam Sardar* Pages 1 - 9 ( 9 )
Background: The silver nanoparticles pose high antibacterial properties against multi drug resistant and non-resistant bacteria. However, bacteria are acquiring resistance against chemically synthesized silver nanoparticles after repeated exposure. Therefore, there is inevitable need to understand the mechanistic behavior of silver nanoparticles.
Objective: In this study, we have done complete proteomic analysis of Escherichia coli after treatment with silver nanoparticles to find out the mechanism of bactericidal action of silver nanoparticles (AgNPs).
Methods: Silver nanoparticles were synthesized using Artemisia annua leaf extract and incubated with Escherichia coli to elucidate the antibacterial assay by determining MIC and effect on growth pattern. Further total genome proteins were isolated from control and silver nanoparticles treated bacteria, which were identify by LC MS and Label free quantification analysis technique.
Results: Total identified proteins were 293 out of which 11 proteins were exclusively present in treated bacteria, these are the proteins mainly expressed in stress conditions. Fold change analysis shows 65 proteins were upregulated where stress proteins are overexpressed while membrane proteins were downregulated.
Conclusions: This study reveals that silver nanoparticles inhibits the expression of cellular proteins and causes cell death. Such study may be helpful in designing of drugs against resistant microbes.
Silver Nanoparticles, Escherichia Coli, Proteomics, Bactericidal, Stress Proteins, Artemisia Annua
Research Group for Advanced Materials & Sustainable Catalysis (AMSC), College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, 310032, Research Group for Advanced Materials & Sustainable Catalysis (AMSC), College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, 310032, Department of Biosciences, Faculty of Natural Sciences, Jamia Millia Islamia, New Delhi