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Proteomic Analysis of Differentially Expressed Proteins in Mycobacterium Tuberculosis-Infected Macrophages

[ Vol. 18 , Issue. 1 ]

Author(s):

Shuang Tian, Dongjun Yang, Qian Long and Min Ling*   Pages 12 - 17 ( 6 )

Abstract:


Background: Mycobacterium tuberculosis (MTB) and Mycobacterium avium (MA) are intracellular parasitic bacteria. The biological characteristics of MA and MTB are very similar and need to be identified.

Objective: The study aims to better understand how MTB survives in macrophages and the different pathogenic mechanisms of MTB and MA.

Methods: The Tandem Mass Tag (TMT) and liquid chromatography-tandem mass spectrometry (LCMS/ MS) were used for analysis of the differentially expressed proteins in MTB-infected macrophages and MA-infected macrophages.

Results: A total of 682 proteins were found to be differentially expressed in MTB-infected cells in comparison with MA-infected cells. Among these, 10 proteins (O60812, P06576, O43660-2, E9PL10, O00442, M0R050, Q9H8H0, Q9BSJ8, P41240 and Q8TD57-3) were down-regulated in MTB-infected cells, and M0R050, O00442, Q9H8H0, O60812 and O43660 are interactive proteins which participate in a multitude of cellular RNA processing.

Conclusion: The five down-regulated proteins (M0R050, O00442, Q9H8H0, O60812 and O43660) might repress the synthesis of some resistant proteins in MTB-infected cells to promote MTB survival in macrophages.

Keywords:

Mycobacterium tuberculosis, Mycobacterium avium, TMT technology, survival, macrophage, proteomic.

Affiliation:

Key Laboratory of Biological Molecular Medicine Research of Guangxi University, Department of Biotechnology, School of Basic Medicine Sciences, Guangxi Medical University, Nanning, Key Laboratory of Biological Molecular Medicine Research of Guangxi University, Department of Biotechnology, School of Basic Medicine Sciences, Guangxi Medical University, Nanning, Key Laboratory of Biological Molecular Medicine Research of Guangxi University, Department of Biotechnology, School of Basic Medicine Sciences, Guangxi Medical University, Nanning, Key Laboratory of Biological Molecular Medicine Research of Guangxi University, Department of Biotechnology, School of Basic Medicine Sciences, Guangxi Medical University, Nanning



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