Van-An Duong, Jeeyun Ahn, Na-Young Han, Jong-Moon Park, Jeong-Hun Mok, Tae Wan Kim and Hookeun Lee* Pages 1 - 10 ( 10 )
Background: Diabetic Retinopathy (DR), one of the major microvascular complications commonly occurring in diabetic patients, can be classified into Proliferative Diabetic Retinopathy (PDR) and Non-Proliferative Diabetic Retinopathy (NPDR). Currently available therapies are only targeted for later stages of the disease in which some pathologic changes may be irreversible. Thus, there is a need to develop new treatment options for earlier stages of DR through revealing pathological mechanisms of PDR and NPDR.
Objective: The purpose of this study was to characterize proteomes of diabetic through quantitative analysis of PDR and NPDR.
Methods: Vitreous body was collected from three groups: control (non-diabetes mellitus), NPDR, and PDR. Vitreous proteins were digested to peptide mixtures and analyzed using LC-MS/MS. MaxQuant was used to search against the database and statistical analyses were performed using Perseus. Gene ontology analysis, related-disease identification, and protein-protein interaction were performed using the differential expressed proteins.
Results: Twenty proteins were identified as critical in PDR and NPDR. The NPDR group showed different expressions of kininogen-1, serotransferrin, ribonuclease pancreatic, osteopontin, keratin type II cytoskeletal 2 epidermal, and transthyretin. Also, prothrombin, signal transducer and activator of transcription 4, hemoglobin subunit alpha, beta, and delta were particularly up-regulated proteins for PDR group. The up-regulated proteins related to complement and coagulation cascades. Statherin was down-regulated in PDR and NPDR compared with the control group. Transthyretin was the unique protein that increased its abundance in NPDR compared with the PDR and control group.
Conclusion: This study confirmed the different expressions of some proteins in PDR and NPDR. Additionally, we revealed uniquely expressed proteins of PDR and NPDR, which would be differential biomarkers: prothrombin, alpha-2-HS-glycoprotein, hemoglobin subunit alpha, beta, and transthyretin.
Non-proliferative diabetic retinopathy, proliferative diabetic retinopathy, vitreous body, proteomics, LC-MS/MS, gene ontology
College of Pharmacy, Gachon University, Incheon, Department of Ophthalmology, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, Seoul, College of Pharmacy, Gachon University, Incheon, College of Pharmacy, Gachon University, Incheon, College of Pharmacy, Gachon University, Incheon, Department of Ophthalmology, SNU Blue Eye Clinic, Seoul, College of Pharmacy, Gachon University, Incheon