Sungkyoung Lee, Myoung-Ro Lee, Songmee Bae and Min-Kyu Kwak* Pages 1 - 15 ( 15 )
Background: Streptococcus pneumoniae is a leading cause of human respiratory tract infection. Despite the lack of activities of antioxidative enzymes, including cytochromes, hemoproteins, and peroxidases/catalases, traits conferring the aerotolerant-anaerobic growth of this bacterium are conserved, with high efficacy of antioxidative actions, in an oxygen-rich environment.
Objective: Through proteome analysis, this study’s intention was to evaluate differentially expressed proteins and/or gene products modeled in a highly virulent strain, S. pneumoniae D39, exogenously-treated with millimolar concentrations of H2O2.
Method: For two-dimensional gel electrophoresis (2-DE) analysis, following one dimensional isoelectric focusing with an immobilized pH gradient of pH 4-7, the most significantly mobilized proteins expressed were separated by SDS-PAGE in the second dimension. With a total of 431 protein spots detected, certain proteins were excised, in-gel trypsin digested, and analyzed by combination with MALDI-TOF and LC-ESI-MS/MS for mass spectrometric peptide mapping and protein identification. Utilizing mass spectrometry analysis of spots excised from 2-DE, the selected protein spots were identified with a variety of databases and MASCOT.
Results: With the aid of comparisons to proteome reference maps, the most differentially expressed 38 proteins, those with approximately 1.4-fold or more increase and/or decrease or with multiple isoforms exhibiting variable pI values, were induced by treatment of exogenous 2 mM H2O2. The identified proteins were seen to be involved in pneumococcal pathogenesis and primary metabolism, amongst others.
Conclusion: This is the first study to convincingly document proteomic information associated with pathophysiological adaptation under the given oxidative conditions, and corresponding potential antioxidative mechanisms, in S. pneumoniae.
High-throughput image analysis, hydrogen peroxide, LC-coupled ESI-MS/MS, MALDI-TOF, oxidative stress, Streptococcus pneumoniae, two-dimensional gel electrophoresis
Division of Bacterial Disease Research and Parasitic Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, 187 Osongsaengmyeong 2-ro, Osong-eup, Cheongju-si, Chungcheongbuk-do, 28160,, Division of Vectors and Parasitic Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, 187 Osongsaengmyeong 2-ro, Osong-eup, Cheongju-si, Chungcheongbuk-do, 28160,, Division of Antimicrobial Resistance, Center for Infectious Disease Research, Korea National Institute of Health; , Department of Food and Nutrition, Institute of Food and Nutrition Science, Eulji University, 553, Sanseong-daero, Seongnam, Gyeonggi-do, 13135