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ELISA-based Protein Arrays: Multiplexed Sandwich Immunoassays

[ Vol. 1 , Issue. 3 ]


Ruo-Pan Huang, Ying Lin, Li-Pai Chen, Weimin Yang and Ruochun Huang   Pages 199 - 210 ( 12 )


Protein arrays with ability to simultaneously detect multiple protein expression levels, protein-protein interactions, protein-DNA or RNA interactions, protein-small molecule interactions and protein modifications will be an excellent tool in biomedical discovery. A variety of technologies have been developed to meet this need. In general, these assay systems can be divided into two major categories: label-based approaches, and sandwich or enzyme linked immunosorbent assay (ELISA)-based approaches. The purpose of this review is to provide an overview of the principles and techniques of ELISA-based protein arrays, with particular emphasis on the use of ELISA-based protein arrays for detection of multiple protein expression levels.


protein arrays, antibody arrays, proteomics, elisa, protein expression, immunoassay, cytokine


Department of Gynecology and Obstetrics, Emory University School of Medicine, 1639 Pierce Drive,Rm. 4217, Atlanta, GA 30322, USA.

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