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ELISA-based Protein Arrays: Multiplexed Sandwich Immunoassays

[ Vol. 1 , Issue. 3 ]

Author(s):

Ruo-Pan Huang, Ying Lin, Li-Pai Chen, Weimin Yang and Ruochun Huang   Pages 199 - 210 ( 12 )

Abstract:


Protein arrays with ability to simultaneously detect multiple protein expression levels, protein-protein interactions, protein-DNA or RNA interactions, protein-small molecule interactions and protein modifications will be an excellent tool in biomedical discovery. A variety of technologies have been developed to meet this need. In general, these assay systems can be divided into two major categories: label-based approaches, and sandwich or enzyme linked immunosorbent assay (ELISA)-based approaches. The purpose of this review is to provide an overview of the principles and techniques of ELISA-based protein arrays, with particular emphasis on the use of ELISA-based protein arrays for detection of multiple protein expression levels.

Keywords:

protein arrays, antibody arrays, proteomics, elisa, protein expression, immunoassay, cytokine

Affiliation:

Department of Gynecology and Obstetrics, Emory University School of Medicine, 1639 Pierce Drive,Rm. 4217, Atlanta, GA 30322, USA.



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