Kazuhiro Nakanishi, Takaharu Sakiyama, Yoichi Kumada, Koreyoshi Imamura and Hiroyuki Imanaka Pages 161 - 175 ( 15 )
Proteome analysis plays a key role in the elucidation of the functions and applications for numerous proteins. For proteome analyses, various microplate- and microarray-based techniques have been developed by a number of researchers. Their intent was to immobilize proteins on the surface of a solid substrate in a site-directed manner while retaining structure and native biological function. In this review, we focus on recent advances in immobilization methodology for proteins/enzymes on a surface, including those using the affinity peptides screened by random peptide library systems. We also discuss applications of the affinity peptide-mediated immobilization method in fields related to proteome analysis, particularly our recent work concerning immunoassay and protein-protein interaction analysis.
Controlled immobilization, site-directed immobilization, proteome analysis, affinity peptide, ELISA, proteinprotein interaction
Div. of Chem. and Biochem., Grad. School of Natural Sci. and Technol., Okayama Univ., 3-1-1 Tsushima-Naka, Okayama 700-8530, Japan.