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Recent Advances in Controlled Immobilization of Proteins onto the Surface of the Solid Substrate and Its Possible Application to Proteomics

[ Vol. 5 , Issue. 3 ]

Author(s):

Kazuhiro Nakanishi, Takaharu Sakiyama, Yoichi Kumada, Koreyoshi Imamura and Hiroyuki Imanaka   Pages 161 - 175 ( 15 )

Abstract:


Proteome analysis plays a key role in the elucidation of the functions and applications for numerous proteins. For proteome analyses, various microplate- and microarray-based techniques have been developed by a number of researchers. Their intent was to immobilize proteins on the surface of a solid substrate in a site-directed manner while retaining structure and native biological function. In this review, we focus on recent advances in immobilization methodology for proteins/enzymes on a surface, including those using the affinity peptides screened by random peptide library systems. We also discuss applications of the affinity peptide-mediated immobilization method in fields related to proteome analysis, particularly our recent work concerning immunoassay and protein-protein interaction analysis.

Keywords:

Controlled immobilization, site-directed immobilization, proteome analysis, affinity peptide, ELISA, proteinprotein interaction

Affiliation:

Div. of Chem. and Biochem., Grad. School of Natural Sci. and Technol., Okayama Univ., 3-1-1 Tsushima-Naka, Okayama 700-8530, Japan.



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